Research Technician III
Fred Hutchinson Cancer Center
- Seattle, WA
- $67,716-99,236 per year
- Permanent
- Full-time
- Organize and assist with the management of a cancer research laboratory including maintaining a biospecimen bank, organizing freezer/liquid nitrogen biospecimen storage, train laboratory personnel in standard methods/assays (tissue culture, PCR, DNA/RNA extractions, organoid development, mouse handling, xenograft implantation and harvesting, xenograft treatments).
- Work collaboratively with other lab members to execute complex experimental studies of cancer therapeutics including assessments of tumor responses in vitro and in vivo.
- Perform cell-culture experiments, molecular biology, and preparation of next-generation sequencing libraries.
- Maintain and perform husbandry and genotyping of murine colonies, as well as, to maintain associated databases
- Histology, cell culture, maintain mice colonies, imaging mice, stocking reagents
- Tag, weigh and record data used for tracking purposes and administer drugs/reagents per guided protocols, including the harvest of brain tumors
- Perform, collect and analyze flow cytometric assays
- Develop and characterize novel antibodies targeting tumor antigens
- IVIS and ultrasound imaging
- Mentor/train high-school, undergraduate and graduate researchers.
- Assist with laboratory supply ordering.
- Attend lab meetings and present results.
- Read relevant scientific literature.
- Assist with general lab upkeep and maintenance.
- Optionally (depending on skills and interests) assisting with computational data analysis and the preparation/authorship of manuscripts/research results
- A bachelor’s degree in a related field
- A bachelor’s degree in a related field
- Senior level position requiring a minimum of eight years related experience
- Proven ability and knowledge necessary to conduct research independently
- Master's degree or equivalent experience
- Experience with mouse experiments and/or husbandry
- Experience and expertise generating tissue/tumor organoids
- Assay validation and optimization experience
- Experience with cytotoxicity and cytokine assays
- Experience with tissue culture of primary cells, DNA/RNA isolation, and experience with molecular assays such as DNA/RNA sequencing, RT-PCR, immunohistochemistry, CRISPR gene editing
- Experience with cell transfections and the isolation and purification of recombinant proteins
- Experience developing and using biospecimen databases
- Experience/familiarity with R or other programming languages is an asset
- Superior organization and communication skills