Research Technician II/III
Fred Hutchinson Cancer Center
- Seattle, WA
- Permanent
- Full-time
- Perform molecular biology and biochemistry experiments on human and animal biospecimens and cell lines
- Perform tissue culture experiments and maintain cultures and reagents in a sterile environment
- Work with antibodies (immunoaffinity purification, immobilization on solid supports, perform ELISA to screen hybridomas)
- Assist in generation of mass spectrometry (MS)-based proteomic data
- Process proteomic samples for LC-MS analysis (e.g., protein extraction, fractionation, immuno-depletion, trypsin digestion, alkylation, reduction, etc.)
- Follow standard operating protocols (SOP) in a CLIA/CAP laboratory environment including participating in annual competency and proficiency testing
- Collaborate effectively with an interdisciplinary team spanning expertise in medicine, biology, analytical chemistry, and statistics
- Maintain laboratory equipment, records, research results files, and databases
- Assist with written progress reports and development of standard operating protocols (SOPs)
- Assist with writing scientific manuscripts (in peer-reviewed journals)
- Perform other duties as required
- B.S. degree and minimum 2+ years post-degree bench experience in Protein Chemistry, Analytical Chemistry, Biochemistry, Molecular Biology or related discipline. Salary and title based on experience.
- The work is rigorously quantitative, and exquisite attention to detail and the ability to adhere to standard operating protocols (SOP) and maintain records is critical.
- The position includes drafting reports and parts of manuscripts for publication, as well as presenting experimental results to the research team and collaborators. Thus, excellent English writing and oral communication skills
- Ability to work as part of an interdisciplinary team
- Proficiency with MS Office programs (Word, Powerpoint, Excel, and Access)
- Ability to multi-task efficiently in a highly interactive team environment
- Must be comfortable in a milestone-driven environment, with quarterly objectives
- Willingness to learn new techniques
- Passion for translating biology into clinical solutions
- Strong work ethic
- Previous tissue culture experience and sterile technique
- Previous experience in a CLIA/CAP or similar clinical lab environment
- Previous work in proteomics, genomics, and functional biology