Senior Scientist I, Cell/Molecular Biology - Cell Line Development
- San Francisco, CA
- Permanent
- Full-time
- Design, test and optimize DNA vector constructs and vector workflows for protein expression (including monoclonal antibodies and antibody-like molecules) in CHO cells. Apply deep knowledge of expression vector design principles to optimize RNA expression levels and assembly of multi-chain biologics.
- Design experiments and perform genetic characterization of CHO cell lines/banks using multiple methods (ddPCR, NGS by Illumina MiSeq and Nanopore PromethION 2, TLA, occasionally Southern/Northern blotting) to confirm identity, quality, and stability of transgene DNA and RNA. Manage and analyze all associated data, including NGS data.
- Optimize genetic characterization workflows for technological advancement, efficiency and timeline improvement. Set up automation of wet lab and data analysis workflows.
- Design and perform experiments for engineering gene knock-in/-out/-down in CHO cells.
- Display effective organization, verbal and written communication skills, and adherence to good documentation practices, including real time entry of lab work into electronic lab notebook.
- Author detailed technical reports that support regulatory filings at all stages from IND to BLA.
- Present updates at team meetings and collaborate closely with others in the cell line development group.
- Bachelor’s Degree with 10 years of relevant experience, OR Master’s Degree with 8 years of relevant experience, OR PhD with no experience.
- Proficiency with molecular biology techniques including complex molecular cloning; PCR, qPCR, ddPCR on DNA and RNA(RT); NGS (MiSeq and PromethION 2 preferred, both library prep and data analysis); gene editing technologies (CRISPR, ZFN, or other).
- Hands-on experience using Illumina and Nanopore sequencers running DNA and RNA-seq workflows, from library prep, to instrument operation, to data analysis and data management.
- Experience with NGS data analysis software, including genome browser (IGV) NGS data file formats, "FASTQC", Samtool, and other related tools to carry out basic NGS data analysis.
- Familiarity with automated liquid handling work stations, such as Hamilton, Tecan, Biomek, or other. Familiarity with writing scripts to set up new automated workflows. Ability to troubleshoot software and hardware issues in collaboration with other specialists.
- Deep understanding of cell and molecular biology concepts underpinning protein expression in CHO cells, including transfection and DNA integration; RNA transcription and its control; protein translation and its control; protein folding, glycosylation and secretion; mechanisms of genotypic instability in CHO cells.
- Proficiency with sterile mammalian suspension cell culture; cell transfection; analytical flow cytometry.
- Independently design experiments with proper controls, sample size and statistical analysis to reach sound conclusions. Independently generate precise, reliable and reproducible data in a timely manner. Demonstrate strong data interpretation skills.
- Excellent attention to detail and ability to keep detailed written records of protocols, raw materials, equipment, etc. in electronic lab notebook on a daily basis.
- Proficiency with the following software: Microsoft Office, Geneious Prime, Spotfire, Sequencher, bioinformatics tools.
- Proficiency in one or more programming/data science languages, such as R, Python, Java, C/C++ . Familiarity with Linux/Unix.
- Ability to learn, understand, and execute new scientific techniques.
- Ability to multi-task and work within timelines.
- Ability to work collaboratively within and outside of group.
- Strong communication skills – verbal, written, scientific data presentation.
- Experience with synthetic biology screens
- Familiarity with Chemistry, Manufacturing and Controls (CMC) and specifically CHO cell line development for GMP applications within biotech/pharma industry. Familiarity with relevant ICH guidelines.