Associate Scientist II orScientist I, CMC Drug Substance Cell Line Development Group
AbbVie
- San Francisco, CA
- Permanent
- Full-time
- Design and generate DNA vector constructs for protein expression (including monoclonal antibodies and antibody-like molecules)
- Optimize the vector workflow to increase speed and throughput
- Perform genetic characterization of CHO cell banks to confirm identity and evaluate quality of transgene DNA and RNA.
- Review DNA sequencing data (including NGS), Southern/Northern blots, quantitative PCR results
- Perform routine molecular screens of CHO cell lines using ddPCR and MinION, P2 sequencers
- Author technical reports that meet expectations of regulatory agencies for all stages from IND to BLA
- Present updates at team meetings and collaborate closely with others in the cell line development group.
- Actively seek opportunities to optimize or enhance the molecular characterization workflow.
- Display effective organization, verbal and written communication skills, and adherence to good documentation practices.
- Proficiency with basic molecular biology techniques (molecular cloning, plasmid preps, DNA digests/gels, PCR/qPCR, DNA sequencing), sterile technique, and mammalian cell culture.
- Strong understanding of mammalian cell biology and molecular biology concepts.
- Independently generate precise, reliable and reproducible data in a timely manner. Demonstrate experimental precision and strong data interpretation skills and should possess understanding of core discipline.
- Excellent attention to detail and ability to keep detailed written records in lab notebook on a daily basis.
- Proficiency with Microsoft Office software, ability to learn new software applications.
- Proficiency with Geneious software.
- Ability to learn, understand, and execute new scientific techniques.
- Ability to multi-task and work within timelines.
- Ability to work collaboratively within and outside of group.
- Strong communication skills – verbal, written, scientific data presentation.
- Background in CHO cell line development for GMP applications within biotech/pharma industry.
- Hands-on experience using Illumina and Nanopore sequencers running RNA-seq workflow and analysis
- Experience with ddPCR
- Experience in bioinformatics software to analyze DNA and RNA for sequence confirmation and expression analysis
- Familiarity with genome browser (IGV) and familiarity with NGS data file formats
- Ability to use bioinformatics tools such as "FASTQC", Samtool, and other related tools to carry out basic NGS analysis
- Proficiency in programming/data science languages, such as R, Python, and Java
- Understanding of cell biology and bioprocess engineering concepts